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Agar plates and pressure cookers

Discussion in 'Science' started by MyOrchid, Feb 10, 2020.

  1. MyOrchid

    MyOrchid Established commenter

    Dear All,

    One of our technicians would like to treat agar plates by pressure-cooking to kill micro-organisms prior to disposal as we don't have an autoclave. We have not done this before and she would greatly appreciate any advice you may have regarding this process in terms of time and temperature required and any other handy hints.

    All information greatly appreciated.

    Thank you
     
  2. border_walker

    border_walker Lead commenter

    CLEAPSS
     
    bonxie likes this.
  3. Skeoch

    Skeoch Star commenter

    @border_walker is exactly right. And if your school/LA isn't a member, the first thing to spend your budget on is .... CLEAPSS.
     
    bonxie and border_walker like this.
  4. MyOrchid

    MyOrchid Established commenter


    Yes, found a great video on their website, thanks. I am not in the UK but I have suggested to the HoD that we join CLEAPSS. Our technician is happy, and has gone off to find suitable bags, thanks.
     
    border_walker likes this.
  5. blazer

    blazer Star commenter

    IIRC a pressure cooker should produce a temp of about 120C. Plastic plates will shrink and buckle.
    Could they be microwaved?
     
  6. Skeoch

    Skeoch Star commenter

    Not pre-empting CLEAPSS' advice - but you buy disposable plasic bags into which all the plastics go. Everything distorts, but that doesn't matter because the whole bag and contents go into normal waste, not biohazard.
    You can't re-sterilise plastic plates.
     
    phlogiston and border_walker like this.
  7. border_walker

    border_walker Lead commenter

    Or use glass ones.
     

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